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New scaffolds for tissue engineering using adipose-derived stem cells
https://doi.org/10.14988/pa.2017.0000013079
https://doi.org/10.14988/pa.2017.000001307991be9f7f-b7fd-425c-be07-257cf9def0c2
名前 / ファイル | ライセンス | アクション |
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023054010007.pdf (714.8 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-05-29 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | New scaffolds for tissue engineering using adipose-derived stem cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | 脂肪組織由来幹細胞, PGA不織布, コラーゲンコーティング, 足場, 親水性への変化 ADSCs, non-woven PGA fabric, collagen-coating, scaffold, change to hydrophilicity |
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資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.14988/pa.2017.0000013079 | |||||
ID登録タイプ | JaLC | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
Kawasumi, Akari
× Kawasumi, Akari× Wang, Zhen× Kotani, Yutaka× Tamura, Atsushi× Tsuji, Misaki× Hayashi, Maho× Ikeda, Junki× Orikasa, Taichi× Takamori, Hideki× Torii, Hiroko× Ozamoto, Yuki× Morita, Shinichiro× Tsujimoto, Hiroyuki× Urabe, Mamoru× Hagiwara, Akeo |
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著者所属(英) | ||||||
en | ||||||
Kawasumi, Akari / Undergraduate Senior, Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Wang, Zhen / Undergraduate Senior, Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Kotani, Yutaka / Undergraduate Senior, Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Tamura, Atsushi / Undergraduate Senior, Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Tsuji, Misaki / Undergraduate Senior, Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Hayashi, Maho / Undergraduate Senior, Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Ikeda, Junki / Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Orikasa, Taichi / Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Takamori, Hideki / Department of Medical Life System, Doshisha University / Research and Development Department, Gunze Ltd | ||||||
著者所属(英) | ||||||
en | ||||||
Torii, Hiroko / Department of Medical Life System, Doshisha University / Department of Kusatsu General Hospital | ||||||
著者所属(英) | ||||||
en | ||||||
Ozamoto, Yuki / Department of Kusatsu General Hospital | ||||||
著者所属(英) | ||||||
en | ||||||
Morita, Shinichiro / Research and Development Department, Gunze Ltd | ||||||
著者所属(英) | ||||||
en | ||||||
Tsujimoto, Hiroyuki / Department of Medical Life System, Doshisha University | ||||||
著者所属(英) | ||||||
en | ||||||
Urabe, Mamoru / Department of Medical Life System, Doshisha University / Department of Kusatsu General Hospital | ||||||
著者所属(英) | ||||||
en | ||||||
Hagiwara, Akeo / Department of Medical Life System, Doshisha University | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ADSCs(脂肪組織由来幹細胞)は再生医療において有用な細胞源であり、臨床でも応用されている。しかしながら三次元的マトリックスの足場なしでは複雑な構造の組織再生は難しく、ADSCsを用いてこのような組織再生を行なう際には足場が必要である。従来、人工足場としてはPGA不織布が汎用されているが、PGAは疎水性であるためにADSCsの接着性と増殖性が低いと考えられる。細胞接着性と増殖性を改善するために、我々はコラーゲンコーティングPGA不織布を開発した。ラットの鼠蹊部より採取した脂肪組織から得た細胞の内でフローサイトメトリーによりCD29、90が陽性かつCD 11b、45が陰性と特徴つけられる細胞群をADSCsとして実験を行った。In Vitroの細胞増殖試験で、コラーゲンコーティングPGA不織布上では、それに対応するコラーゲン非コーティングPGA不織布上よりも細胞の接着・増殖が良好であった。この結果は、コラーゲンコーティングによりPGA不織布繊維表面が親水性になり、ADSCsの接着・増殖に有利な環境に変化したためと考えられる。 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Adipose-derived stem cells (ADSCs) have attracted attention as a useful cell source for tissue engineering, even in the clinical setting. However, it is difficult to regenerate tissues with complex structures in the absence of a three-dimensional matrix such as an artificial scaffold. Namely, scaffolds are required to allow implanted ADSCs to regenerate tissues with three-dimensional structures. Although non-woven PGA (poly (glycolic acid)) fabric has been used widely as an artificial scaffold, ADSCs poorly adhere to and grow on PGA fabric due to its hydrophobicity. To improve cell adhesion and growth on PGA fibers, we devised a PGA fabric coated with collagen. We isolated ADSCs from the inguinal adipose tissue of a rat and characterized the cells using flow cytometry with both CD29- and CD90-positive markers and CD11b- and CD45-negative markers. Our cell growth examination revealed that ADSC growth was improved on all of the collagen-coated fabrics compared with that observed on the corresponding non-coated fabrics. This may be because ADSCs are able to adhere and grow more readily on the hydrophilic surface of collagen-coated PGA fabrics. | |||||
言語 | en | |||||
書誌情報 |
ja : 同志社大学理工学研究報告 en : The Science and Engineering Review of Doshisha University 巻 54, 号 1, p. 41-51, 発行日 2013-04-30 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 同志社大学理工学研究所 | |||||
出版者(英) | ||||||
言語 | en | |||||
出版者 | Science and Engineering Research Institute of Doshisha University | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 00368172 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00165868 | |||||
権利者情報 | ||||||
権利者名 | 同志社大学理工学研究所 | |||||
言語 | ja | |||||
権利者名 | Science and Engineering Research Institute of Doshisha University | |||||
言語 | en | |||||
関連サイト | ||||||
関連タイプ | isFormatOf | |||||
識別子タイプ | URI | |||||
関連識別子 | https://doors.doshisha.ac.jp/opac/opac_link/bibid/SB00960326/?lang=0 | |||||
言語 | ja | |||||
関連名称 | 掲載刊行物所蔵情報へのリンク / Link to Contents | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題 | 464.26 |