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DNAプローブ固定化微粒子を用いたDNA一塩基変異検出法の開発
https://doi.org/10.14988/pa.2017.0000012464
https://doi.org/10.14988/pa.2017.0000012464f0f7efbd-d3b3-4c2e-83ab-01c1ca86cdac
名前 / ファイル | ライセンス | アクション |
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023052020001.pdf (329.8 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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公開日 | 2011-08-22 | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | ja | |||||||||||||||||
タイトル | DNAプローブ固定化微粒子を用いたDNA一塩基変異検出法の開発 | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | ja-Kana | |||||||||||||||||
タイトル | DNA プローブ コテイカ ビリュウシ オ モチイタ DNA イチエンキ ヘンイ ケンシュツホウ ノ カイハツ | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | en | |||||||||||||||||
タイトル | Development of DNA point mutation detection methods using probe DNA-immobilized microbeads | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | jpn | |||||||||||||||||
キーワード | ||||||||||||||||||
主題 | リガーゼ検出反応, DNA変異, 微粒子, ハイブリダイゼーション ligase detection reaction (LDR), DNA mutation, microbeads, hybridization |
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資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | departmental bulletin paper | |||||||||||||||||
ID登録 | ||||||||||||||||||
ID登録 | 10.14988/pa.2017.0000012464 | |||||||||||||||||
ID登録タイプ | JaLC | |||||||||||||||||
アクセス権 | ||||||||||||||||||
アクセス権 | open access | |||||||||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||
著者 |
橋本, 雅彦
× 橋本, 雅彦
WEKO
16205
× 玉井, 祐介× 塚越, 一彦
WEKO
15597
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著者所属 | ||||||||||||||||||
ja | ||||||||||||||||||
橋本, 雅彦 / 同志社大学理工学部化学システム創成工学科准教授 | ||||||||||||||||||
著者所属 | ||||||||||||||||||
ja | ||||||||||||||||||
玉井, 祐介 / Former undergraduate student of Doshisha University | ||||||||||||||||||
著者所属 | ||||||||||||||||||
ja | ||||||||||||||||||
塚越, 一彦 / 同志社大学理工学部化学システム創成工学科教授 | ||||||||||||||||||
著者所属(英) | ||||||||||||||||||
en | ||||||||||||||||||
Doshisha University | ||||||||||||||||||
所属機関識別子種別 | ||||||||||||||||||
値 | kakenhi | |||||||||||||||||
所属機関識別子 | ||||||||||||||||||
値 | 34310 | |||||||||||||||||
抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | 正常型DNAから微量の変異DNAを識別する手法の一つとして,ポリメラーゼ連鎖反応(PCR)/リガーゼ検出反応(LDR)が知られている.PCR/LDR反応によって得られたターゲットであるLDRプロダクトは,マイクロアレイやゲル電気泳動により分析することができる.本研究では,ターゲット-プローブ間のハイブリダイゼーションにより蛍光標識されたLDRプロダクトを選択的に捕捉するためにプローブDNAが固定化された磁性微粒子を用いた.ハイブリダイゼーションの後,微粒子を洗浄しキャピラリーチューブに導入し,磁石を用いてチューブ内の一カ所に堆積させた.蛍光あるいは化学発光検出を適用することによりチューブ内の微粒子集合体から発光シグナルが得られ,本法がDNA変異検出に適用可能であることが示された. | |||||||||||||||||
言語 | ja | |||||||||||||||||
抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | One technique that can distinguish low-abundant mutant DNA from wild-type DNA is the ligase detection reaction (LDR) coupled to a primary polymerase chain reaction (PCR). The LDR products as a target obtained by the sequential PCR/LDR reactions can be analyzed in a variety of fashions such as microarray and slab gel electrophoresis. In the present work, we employed probe DNA-immobilized magnetic microbeads to selectively capture the fluorescently labeled-LDR products via the target hybridization with the probe. After the hybridization, the microbeads were rinsed and introduced into a capillary tube and packed in one space in the tube with a magnet. Photoluminescence from the beads aggregates in the tube was successfully obtained with fluorescence or chemiluminescence detection system, showing that the present method can be amenable to mutation detection. | |||||||||||||||||
言語 | en | |||||||||||||||||
書誌情報 |
ja : 同志社大学理工学研究報告 en : The Science and Engineering Review of Doshisha University 巻 52, 号 2, p. 93-98, 発行日 2011-07-31 |
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出版者 | ||||||||||||||||||
言語 | ja | |||||||||||||||||
出版者 | 同志社大学理工学研究所 | |||||||||||||||||
出版者(英) | ||||||||||||||||||
言語 | en | |||||||||||||||||
出版者 | Science and Engineering Research Institute of Doshisha University | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 00368172 | |||||||||||||||||
書誌レコードID | ||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AN00165868 | |||||||||||||||||
権利者情報 | ||||||||||||||||||
権利者名 | 同志社大学理工学研究所 | |||||||||||||||||
言語 | ja | |||||||||||||||||
権利者名 | Science and Engineering Research Institute of Doshisha University | |||||||||||||||||
言語 | en | |||||||||||||||||
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関連タイプ | isFormatOf | |||||||||||||||||
識別子タイプ | URI | |||||||||||||||||
関連識別子 | https://doors.doshisha.ac.jp/opac/opac_link/bibid/SB00960326/?lang=0 | |||||||||||||||||
言語 | ja | |||||||||||||||||
関連名称 | 掲載刊行物所蔵情報へのリンク / Link to Contents | |||||||||||||||||
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内容記述タイプ | Other | |||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||
出版タイプ | ||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
日本十進分類法 | ||||||||||||||||||
主題 | 433.9 |