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キャピラリーゲル電気泳動によるリガーゼ検出反応生成物の分析
https://doi.org/10.14988/pa.2017.0000012060
https://doi.org/10.14988/pa.2017.0000012060e17864b4-c10c-4164-92de-fa4513d4e715
名前 / ファイル | ライセンス | アクション |
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023050040002.pdf (278.5 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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公開日 | 2010-03-01 | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | ja | |||||||||||||||||
タイトル | キャピラリーゲル電気泳動によるリガーゼ検出反応生成物の分析 | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | ja-Kana | |||||||||||||||||
タイトル | キャピラリーゲル デンキ エイドウ 二ヨル リガーゼ ケンシュツ ハンノウ セイセイブツ ノ ブンセキ | |||||||||||||||||
タイトル | ||||||||||||||||||
言語 | en | |||||||||||||||||
タイトル | Capillary gel electrophoresis for ligase detection reaction products | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | jpn | |||||||||||||||||
キーワード | ||||||||||||||||||
主題 | キャピラリーゲル電気泳動, ポリマー溶液, 点突然変異, リガーゼ検出反応 capillary gel electrophoresis, polymer solution, point mutation, ligase detection reaction |
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資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | departmental bulletin paper | |||||||||||||||||
ID登録 | ||||||||||||||||||
ID登録 | 10.14988/pa.2017.0000012060 | |||||||||||||||||
ID登録タイプ | JaLC | |||||||||||||||||
アクセス権 | ||||||||||||||||||
アクセス権 | open access | |||||||||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||
著者 |
橋本, 雅彦
× 橋本, 雅彦
WEKO
16205
× 上郡, 純× 塚越, 一彦
WEKO
15597
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著者所属 | ||||||||||||||||||
ja | ||||||||||||||||||
橋本, 雅彦 / Department of Chemical Engineering and Materials Science, Doshisha University | ||||||||||||||||||
著者所属 | ||||||||||||||||||
ja | ||||||||||||||||||
上郡, 純 / Former graduate student of Doshisha University | ||||||||||||||||||
著者所属 | ||||||||||||||||||
ja | ||||||||||||||||||
塚越, 一彦 / Department of Chemical Engineering and Materials Science, Doshisha University | ||||||||||||||||||
著者所属(英) | ||||||||||||||||||
en | ||||||||||||||||||
Doshisha University | ||||||||||||||||||
所属機関識別子種別 | ||||||||||||||||||
値 | kakenhi | |||||||||||||||||
所属機関識別子 | ||||||||||||||||||
値 | 34310 | |||||||||||||||||
抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | 正常型DNAから微量の変異DNAを識別する手法の一つとして,ポリメラーゼ連鎖反応(PCR)/リガーゼ検出反応(LDR)が知られている.このPCR/LDRアッセイによって得られたLDR生成物は,マイクロアレイやスラブゲル電気泳動によって分析されるのが一般的である.本研究で,筆者らは,LDR生成物の分析のために,キャピラリーゲル電気泳動(CGE)-蛍光(FL)検出システムを適用した.CGE-FL分析システムにおいて,一本鎖DNAとも結合し蛍光を発するSYBR Goldを分離マトリックスであるポリエチレンオキシド(PEO)ポリマー溶液に添加し,分析を行った.本分析システムにおいて,LDR生成物をLDRプライマーやテンプレートなどの他のDNAフラグメントから分離・検出することが可能となり,K-ras遺伝子のコドン12における一塩基置換の識別が達成された. | |||||||||||||||||
言語 | ja | |||||||||||||||||
抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | One technique that can distinguish low-abundant mutant DNA from wild-type DNA is the ligase detection reaction (LDR) coupled to a primary polymerase chain reaction (PCR). The LDR products obtained by the PCR/LDR assay can be analyzed in a variety of fashions such as microarray and slab gel electrophoresis. In the present work, we applied capillary gel electrophoresis (CGE) with fluorescence detection (FL) (CGE-FL) for analyzing the LDR products. Polyethylene oxide (PEO) polymer solution containing SYBR Gold, which could bind to a single strand DNA, was used as a separation matrix in the CGE-FL system. The LDR products could successfully be separated from other DNA fragments such as LDR primers and template, enabling the determination of the single base substitutions on codon 12 of K-ras gene. | |||||||||||||||||
言語 | en | |||||||||||||||||
書誌情報 |
ja : 同志社大学理工学研究報告 en : The Science and Engineering Review of Doshisha University 巻 50, 号 4, p. 6-12, 発行日 2010-01-31 |
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出版者 | ||||||||||||||||||
言語 | ja | |||||||||||||||||
出版者 | 同志社大学理工学研究所 | |||||||||||||||||
出版者(英) | ||||||||||||||||||
言語 | en | |||||||||||||||||
出版者 | Science and Engineering Research Institute of Doshisha University | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 00368172 | |||||||||||||||||
書誌レコードID | ||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AN00165868 | |||||||||||||||||
権利者情報 | ||||||||||||||||||
権利者名 | 同志社大学理工学研究所 | |||||||||||||||||
言語 | ja | |||||||||||||||||
権利者名 | Science and Engineering Research Institute of Doshisha University | |||||||||||||||||
言語 | en | |||||||||||||||||
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関連タイプ | isFormatOf | |||||||||||||||||
識別子タイプ | URI | |||||||||||||||||
関連識別子 | https://doors.doshisha.ac.jp/opac/opac_link/bibid/SB00960326/?lang=0 | |||||||||||||||||
言語 | ja | |||||||||||||||||
関連名称 | 掲載刊行物所蔵情報へのリンク / Link to Contents | |||||||||||||||||
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内容記述タイプ | Other | |||||||||||||||||
内容記述 | application/pdf | |||||||||||||||||
出版タイプ | ||||||||||||||||||
出版タイプ | VoR | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
日本十進分類法 | ||||||||||||||||||
主題 | 433.9 |