@article{oai:doshisha.repo.nii.ac.jp:00021475,
 author = {堀, 未央 and Hori, Mio and 八木, 雅之 and Yagi, Masayuki and 埜本, 慶太郎 and Nomoto, Keitaro and 北野, 貴大 and Kitano, Takahiro and 宮崎, 亮 and Miyazaki, Ryo and Yonei, Yoshikazu and 米井, 嘉一},
 issue = {3},
 journal = {同志社大学理工学研究報告, The Science and Engineering Review of Doshisha University},
 month = {Oct},
 note = {グルコースと蛋白質の非酵素的な糖化反応により,蛋白糖化最終生成物(advanced glycation end products: AGEs)が生成される.AGEsの生体内蓄積は糖尿病合併症や加齢に伴う疾患に関与しているため,近年では抗糖化は健康増進,疾患予防,アンチエイジングなどの分野で注目されている。本研究ではクマイザサ抽出物(Sasa senanensis Rehder)の抗糖化作用を検討し,「抗糖化」をキーワードとした健康食品・化粧品素材しての可能性を模索することを目的とした.今回,ヒト血清アルブミン(human serum albumin; HSA)とグルコースを反応させたin vitro実験系において,蛍光性AGEs,3-デオキシグルコソン(3DG),ペントシジン(Pent),カルボキシメチルリジン(CML)をAGEsとして評価し,陽性対照として塩酸アミノグアニジン(AG)を用い,蛍光測定法・ELISA法・HPLC法にてAGEsの測定を行った。抗糖化活性としてIC50(50%生成阻害濃度)を算出した。蛍光性AGEsに関してクマイザサ(粉末)の抗糖化活性のIC50はAGと同等であった.クマイザサ(熱水抽出物)の抗糖化活性のIC50はAGと比べ極めて小さかった.3DG生成阻害活性に関して,クマイザサ(熱水抽出物)のIC50はアミノグアニジンと同等であった.クマイザサ(粉末)のIC50はAGよりも極めて小さかった.Pent生成阻害活性に関して,AGと比較してクマイザサ(粉末)および(熱水抽出物)のIC50は極めて小さかった.CML生成阻害率に関して,AGと比較してクマイザサ(粉末)および(熱水抽出物)のIC50は極めて小さかった.クマイザサについては初めて糖化物生成抑制作用が確認され,健康食品・化粧品素材としての可能性が期待される., Non-enzymatic reaction between glucose and protein leads to the formation of advanced glycation end products (AGEs), and its accumulation has been linked to the development of diabetic complications, as well as to the progression of age-related diseases. In recent years, attention has been paid to the effect of inhibiting AGE formation in the body for the purposes of anti-aging, health promotion, and lifestyle-disease prevention. In the present study, we evaluated the anti-glycation effects of Kumaizasa (Sasa senanensis Rehder) and the potential of its use as an anti-glycation product. Using an in vitro method with glucose and human serum albumin (HSA), we analyzed the inhibition of the formation of AGEs; specifically fluorescent AGEs, 3-deoxyglucosone (3DG), Pentosidine (Pent), and Nε-(carboxymethyl) lysine (CML) by Kumaizasa utilizing fluorescence spectroscopy, HPLC, and enzyme linked immunoassay (ELISA). We analyzed its anti-glycation effects by comparing the 50% inhibitory concentration (IC50) against these glycated products with aminoguanidine, a known inhibitor of glycation. IC50 of fluorescent AGEs by Kumaizasa (powder form) and aminoguanidine were almost equal concentrations, while IC50 against 3DG, Pent, and CML were significantly lower for Kumaizasa (powder form) than that of aminoguanidine. IC50 of 3DG by Kumaizasa (hot water extraction) and aminoguanidine were almost equal concentrations, while IC50 against fluorescent AGEs, Pent, and CML were significantly lower for Kumaizasa (hot water extraction) than that of aminoguanidine. These results suggest that Kumaizasa strongly inhibits glycation and may be useful for anti-glycation products, including health foods and cosmetics., application/pdf},
 pages = {223--229},
 title = {クマイザサ(Sasa senanensis Rehder)の蛋白糖化最終生成物(AGEs)生成抑制作用の研究},
 volume = {52},
 year = {2011},
 yomi = {ホリ, ミオ and ヤギ, マサユキ and ノモト, ケイタロウ and キタノ, タカヒロ and ミヤザキ, リョウ and ヨネイ, ヨシカズ}
}