{"created":"2023-07-27T07:30:50.461753+00:00","id":1624,"links":{},"metadata":{"_buckets":{"deposit":"e90e76b2-3db0-454e-af65-a4b9f7022ad2"},"_deposit":{"created_by":20,"id":"1624","owners":[20],"pid":{"revision_id":0,"type":"depid","value":"1624"},"status":"published"},"_oai":{"id":"oai:doshisha.repo.nii.ac.jp:00001624","sets":["4151:8699:4203:4204","4251:7687:7688:8785:7689"]},"author_link":["3508"],"item_1693813220986":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1694497428290":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(理学)","subitem_degreename_language":"ja"}]},"item_1694497507610":{"attribute_name":"学位名（英）","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy in Science","subitem_degreename_language":"en"}]},"item_1694497573527":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"同志社大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Doshisha University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34310","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_3_alternative_title_2":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"シヌクレイノパチー脳におけるα-シヌクレイン凝集体の増幅と増幅後の構造特性","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"シヌクレイノパチー ノウ ニオケル α-シヌクレイン ギョウシュウタイ ノ ゾウフク ト ゾウフクゴ ノ コウゾウ トクセイ","subitem_alternative_title_language":"ja-Kana"}]},"item_3_date_granted_27":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-03-22"}]},"item_3_description_12":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"神経変性疾患で蓄積する異常タンパク質の1つであるα-synは、PD、DLBおよびMSAの脳内に主に蓄積する。DLBやMSAの患者脳から解析可能な量のα-syn凝集体の増幅に成功した。増幅前後の凝集体のプロテイナーゼKコアのMS分析結果から、増幅による変化はないもののマウスとヒトのα-syn凝集体で切断パターンが異なることがわかった。これらの結果から、この方法が神経変性疾患の異常タンパク質研究の発展に貢献できることを示唆した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Pathological proteins related to neurodegenerative diseases are misfolded, aggregating to form amyloid fibrils. One of the pathological proteins, α-syn, accumulates in the brains of PD, DLB and MSA. We first performed amplification of α-syn aggregates. We successfully amplified enough α-syn aggregates derived from α-syncleinopathies. We found that the MS analysis results of proteinase K-resistant cores of the aggregates before and after the amplification differ between mouse and human α-syn aggregates. The results suggest that structural properties of amplified α-syn fibrils are preserved and these methods can be applicable in the study of pathological proteins of the neurodegenerative disorders.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_dissertation_number_28":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第1095号"}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14988/di.2020.0000000185","subitem_identifier_reg_type":"JaLC"}]},"item_3_relation_16":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"BC02444353","subitem_relation_type_select":"NCID"}}]},"item_3_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"掲載刊行物所蔵情報へのリンク ／ Link to Contents"}],"subitem_relation_type":"isFormatOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doors.doshisha.ac.jp/opac/opac_link/bibid/BB13127448/?lang=0","subitem_relation_type_select":"URI"}}]},"item_3_text_8":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"脳科学研究科"}]},"item_3_text_9":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Graduate School of Brain Science"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉永, 早希","creatorNameLang":"ja"},{"creatorName":"ヨシナガ, サキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshinaga, Saki","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-28"}],"displaytype":"detail","filename":"k1095.pdf","filesize":[{"value":"327.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨/abstaract","objectType":"abstract","url":"https://doshisha.repo.nii.ac.jp/record/1624/files/k1095.pdf"},"version_id":"5753d33c-cda2-4718-9f0e-f9a0450fa16d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-01"}],"displaytype":"detail","filename":"zk1095.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"zk1095.pdf","objectType":"fulltext","url":"https://doshisha.repo.nii.ac.jp/record/1624/files/zk1095.pdf"},"version_id":"db5afdbc-0a5a-4d04-92bb-f6c244347adc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"alpha-synuclein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"synucleinopathy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"seeding reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein misfolding cyclic amplification(PMCA)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mass spectrometry","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Preserved structural property after amplification of alpha-synuclein aggregates from brains of synucleinopathies","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preserved structural property after amplification of alpha-synuclein aggregates from brains of synucleinopathies","subitem_title_language":"en"}]},"item_type_id":"3","owner":"20","path":["4204","7689"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-01"},"publish_date":"2020-06-01","publish_status":"0","recid":"1624","relation_version_is_last":true,"title":["Preserved structural property after amplification of alpha-synuclein aggregates from brains of synucleinopathies"],"weko_creator_id":"20","weko_shared_id":-1},"updated":"2023-12-20T00:30:05.829843+00:00"}