{"created":"2023-07-27T07:30:43.805115+00:00","id":1486,"links":{},"metadata":{"_buckets":{"deposit":"442d3a48-e99a-4838-a1ae-a888f42e4d23"},"_deposit":{"created_by":20,"id":"1486","owners":[20],"pid":{"revision_id":0,"type":"depid","value":"1486"},"status":"published"},"_oai":{"id":"oai:doshisha.repo.nii.ac.jp:00001486","sets":["4151:8699:4190:4191","4251:7209:7210:8775:7211"]},"author_link":["3257"],"item_1693813220986":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1694497428290":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(工学)","subitem_degreename_language":"ja"}]},"item_1694497507610":{"attribute_name":"学位名（英）","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy in Engineering","subitem_degreename_language":"en"}]},"item_1694497573527":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"同志社大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Doshisha University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"34310","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_3_alternative_title_2":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ゲノムサイズDNAの二本鎖損傷と構造転移研究","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"ゲノム サイズ DNA ノ ニホンサ ソンショウ ト コウゾウ テンイ ケンキュウ","subitem_alternative_title_language":"ja-Kana"}]},"item_3_date_granted_27":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-09-20"}]},"item_3_description_12":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA中の二本鎖切断(DSB)に対するアスコルビン酸(AA)およびDMSOの保護効果を、蛍光顕微鏡による巨大DNA(T4 DNA; 166kbp)の単分子観察によって評価した。凍結／解凍の状態に対して3つの異なる形態の放射源、可視光、γ線、および超音波の環境下にさらした。1‐プロパノールと2‐プロパノールの間で異なる効果が表れた。ゲノムDNA分子の高次構造の変化は、1−プロパノールを用いると、長軸長が濃度60％で最小を示し、次にアルコール含有量の増加と共に増加する傾向があることを見出した。一方、2−プロパノールを用いると、長軸長はアルコール含有量の増加と共にほぼ単調な減少を示した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"The protective effect of ascorbic acid (AA) and DMSO against double-strand breaks (DSBs) in DNA was evaluated by single-molecule observation of giant DNA (T4 DNA; 166kbp) through fluorescence microscopy. Samples were exposed to three different forms of radiation: visible light, γ-ray, and ultrasound or freeze/thawing. The change of the higher-order structure of genomic DNA molecules in the presence of alcohols by use of single DNA observation with fluorescence microscopy, by focusing our attention to unveil the different effect between 1-propanol and 2-propanol.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_dissertation_number_28":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第969号"}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14988/di.2019.0000000285","subitem_identifier_reg_type":"JaLC"}]},"item_3_relation_16":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"BB28799007","subitem_relation_type_select":"NCID"}}]},"item_3_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"掲載刊行物所蔵情報へのリンク ／ Link to Contents"}],"subitem_relation_type":"isFormatOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doors.doshisha.ac.jp/opac/opac_link/bibid/BB13097333/?lang=0","subitem_relation_type_select":"URI"}}]},"item_3_text_8":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"生命医科学研究科"}]},"item_3_text_9":{"attribute_name":"著者所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Graduate School of Life and Medical Sciences"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ma, Yue","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-10-10"}],"displaytype":"detail","filename":"k969.pdf","filesize":[{"value":"253.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨/abstaract","objectType":"abstract","url":"https://doshisha.repo.nii.ac.jp/record/1486/files/k969.pdf"},"version_id":"36be9e03-f6c0-409b-a7f1-4563a3bad846"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-19"}],"displaytype":"detail","filename":"zk969.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"zk969.pdf","objectType":"fulltext","url":"https://doshisha.repo.nii.ac.jp/record/1486/files/zk969.pdf"},"version_id":"afde58f2-a71d-4419-89d3-13cdff09b20c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Double-strand breaks","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"genome-sized DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high-order structure","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"secondary structure","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Double-strand breaks (DSBs) and structure transition on genome-sized DNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Double-strand breaks (DSBs) and structure transition on genome-sized DNA","subitem_title_language":"en"}]},"item_type_id":"3","owner":"20","path":["4191","7211"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-10-12"},"publish_date":"2018-10-12","publish_status":"0","recid":"1486","relation_version_is_last":true,"title":["Double-strand breaks (DSBs) and structure transition on genome-sized DNA"],"weko_creator_id":"20","weko_shared_id":-1},"updated":"2023-12-20T07:06:24.492494+00:00"}